Journal: Cell reports

Article Title: The IRAK1/IRF5 axis initiates IL-12 response by dendritic cells and control of Toxoplasma gondii infection

doi: 10.1016/j.celrep.2024.113795

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The following antibodies and dilutions were used: IRAK1 (Cell Signaling, 4504S, 1:1000), IRAK2 (Abcam, ab62419, 1:500), IRAK4 (Novus, NB500–597, 1:1000), MyD88 (R&D Systems, AF3109, 1:500), rodent-specific IRF5 (Cell Signaling, 4950, 1:1000), USF2 (Novus Biologicals, NBP1–92649, 1:2000), c-Rel (Cell Signaling, 67489, 1:1000), phospho-IκB-α (Ser32) (Cell Signaling, 2859, 1:750), IκB-α (Cell Signaling, 4812S, 1:1000), phospho-RelA/NF-κB (Ser 536) (Cell Signaling, 3033, 1:1000), RelA/NF-κB (Novus Biologicals, NB100–2176, 1:1000), Actin (Sigma-Aldrich, A2066, 1:3000), phospho-SAPK/JNK (Thr 183/Tyr 185) (Cell Signaling, 4668T, 1:1000), phospho-p38 MAPK (Thr 180/Tyr 182) (Cell Signaling, 4511T, 1:1000), phospho-p44/42 MAPK (Erk 1/2) (Thr 202/Tyr 204) (Cell Signaling, 4370T, 1:2000), phosphor-IKK (Ser 176/180) (Cell Signaling, 2697, 1:1000), CNBP (Santa Cruz, sc-515387-X, 1:200), anti-Goat-IgG HRP-conjugated (R&D Systems, HAF017, 1:5000), anti-Rabbit-IgG HRP-conjugated (Sigma-Aldrich, A0545, 1:5000), anti-Mouse-IgG HRP-conjugated (Cell Signaling, 7076S, 1:5000).

Techniques: Recombinant, Modification, Saline, Protease Inhibitor, Marker, Western Blot, Extraction, Enzyme-linked Immunosorbent Assay, Software

Journal: Artificial cells, nanomedicine, and biotechnology

Article Title: Silence of lncRNA MIAT protects ATDC5 cells against lipopolysaccharides challenge via up-regulating miR-132.

doi: 10.1080/21691401.2019.1626410

Figure Lengend Snippet: Figure 4. Suppressive effect of MIAT silence on the activation of NF-jB and JNK pathways via miR-132. ATDC5 cells were co-transfected with sh-MIAT and miR-132 inhibitor and then subjected to 6 lg/mL LPS. The phosphor/total (p/t) levels of (A,B) p65, IjBa and (C,D) JNK were measured using western blot (n ¼ 3). p < .05 (ANOVA combined with Duncan post-hoc test).

Article Snippet: Anti-caspase-3 (orb378617), anti-cleavedcaspase-3 (orb106556), anti-caspase-9 (orb135175), anticleaved-caspase-9 (orb227889), anti-PARP (orb526607), anti-cleaved-PARP (orb106557), anti-IL-6 (orb6210), anti-IL-8 (orb39299), anti-TNF-a (orb475251), anti-MCP-1 (orb97456), anti-p65 (orb344389), anti-p-p65 (orb14753), anti-IjBa (orb338946), anti-p-IjBa (orb99312), anti-JNK (orb38050), anti-p-JNK (orb184488), and anti-b-actin (orb86987) all from Biorbyt (San Francisco, CA).

Techniques: Activation Assay, Transfection, Western Blot

Journal: PLoS ONE

Article Title: Early effect of laser irradiation in signaling pathways of diabetic rat submandibular salivary glands

doi: 10.1371/journal.pone.0236727

Figure Lengend Snippet: A. Western blot analysis for RAGE (n = 7) and phosphorylated NFκB (n = 4). The bands were detected and quantified using an image analyzer, and RAGE and NFκB protein levels were normalized to that of β-actin. Protein sizes were evaluated by standard protein markers, and their sizes were as follows: RAGE (45 kDa) and phosphorylated p65 (65 kDa). From left to right: control (C0), diabetes (D0) and diabetes treated with LPLI (D20). Bar graphs indicate means ± SE; * P < 0.05 vs control group (C0). B. Immunohistochemistry analysis for RAGE and NFκB in SMG (n = 3). Arrows show the positive staining in the nucleus of excretory ducts cells and perivascular connective tissue cells. Scale bar, 50μM. ED, excretory duct; SD, striated duct; CGT, convoluted granular tubules; VS, blood vessel.

Article Snippet: For inflammatory markers, rabbit monoclonal anti-HMGB1 (1:250; Abcam, Inc., Cambridge, MA, USA, Cat# ab79823, RRID:AB_1603373), rabbit polyclonal TNF-α (IHC World, LLC, Woodstock, MD, Cat #IW-PA1079), rabbit polyclonal anti-RAGE (1:20; GeneTex, Inc., Irvine, CA, USA, Cat# GTX23611, RRID:AB_385109) and rabbit polyclonal anti-phosphorylated NFκB p65 (1:100; Bioss, Inc., Woburn, MA, USA, Bioss, Cat# bs-0982R, RRID:AB_10856012) were used as the primary antibodies.

Techniques: Western Blot, Immunohistochemistry, Staining

Journal: Neurochemical Research

Article Title: Blocking Two-Pore Domain Potassium Channel TREK-1 Inhibits the Activation of A1-Like Reactive Astrocyte Through the NF-κB Signaling Pathway in a Rat Model of Major Depressive Disorder

doi: 10.1007/s11064-023-03857-4

Figure Lengend Snippet: Primary antibodies used in experiments

Article Snippet: P-NF-κB p65 , Rabbit , Bioss , bs-3543R , WB (1:1000)/IF (1:200).

Techniques: Concentration Assay